Pep Guardiola got five players off his unwanted list, while Chelsea completed the most expensive transfer of Deadline Day - read all the latest updates in our transfer round-up.
Read: 10 Transfers That Could Happen Before Transfer Deadline
Young Belgium defender Jason Denayer was the final departure of Guardiola on Deadline Day, following the likes of Joe Hart, Wilfried Bony, Samir Nasri and Eliaquim Mangala through the exit door - all five only completed loan deals though.
Meanwhile, Spurs completed the signing of France midfielder Moussa Sissoko in the final minutes of Wednesday. The midfielder became the most second expensive signing of the day, with Spurs sending £30 million to Newcastle for his services.
Chelsea completed the biggest transfer of Wednesday after brining David Luiz for a reported fee of £34 million back from Paris Saint-Germain.
Leicester City was one of 13 Premier League sides, who broke their club transfer record, after signing Algerian striker Islam Slimani from Sporting. Find all major confirmed deals in our list below.
Confirmed Deals:
Moussa Sissoko (Newcastle to Spurs)
CONFIRMED: We are delighted to announce the signing of @MoussaSissoko from Newcastle United. #SissokoSigns ?? pic.twitter.com/eoA5QAaEsJ
— Tottenham Hotspur (@SpursOfficial) August 31, 2016
David Luiz (PSG to Chelsea)
Welcome back, @DavidLuiz_4!
— Chelsea FC (@ChelseaFC) August 31, 2016
Full story ?? https://t.co/KduBiKbu0j pic.twitter.com/1BoKLG6W0d
Jason Denayer (Man City to Sunderland
CONFIRMED: Jason Denayer has signed for #SAFC on a season-long loan from @ManCity - https://t.co/UPCI96UtlJ pic.twitter.com/nlb91vu79a
Lazar Markovic (Liverpool to Sporting Lisbon) loan
Lazar Markovic has joined @sporting_cp on a season-long loan deal: https://t.co/HjkdXuSW2v pic.twitter.com/CVDdwuUHvM
Hull City have continued with Deadline Day shopping and have brought in Dieumerci Mbokani on a season-long loan.
Enner Valencia has completed a season-long loan move to Ronald Koeman's Everton.
Islam Slimani (Sporting Lisbon to Leicester City)
? DONE DEAL! Algeria striker @slimaniislam completes a #DeadlineDay move to Leicester City. #WelcomeSlimani pic.twitter.com/uA9reEVsjd
West Bromwich Albion have signed Cameroon international, Allan Nyom from Watford.
Hull City have completed the signing of Manchester United midfielder, James Wier on a three-year deal.
Mario Balotelli (Liverpool to OGC Nice)
#BenvenutoMario pic.twitter.com/gxwatH7xDm
Tottenham Hotspur have completed the signing of Pau Lopez on a season-long loan from Espanyol.
David Luiz (Paris Saint-Germain to Chelsea)
PSG agree move to send @DavidLuiz_4 back to Chelsea https://t.co/mEuZxz0siU pic.twitter.com/EbzOgTlkVl
Marseille winger, Georges-Kévin N'Koudou has joined Premier League outfit, Tottenham Hotspur.
Juan Cuadrado (Chelsea to Juventus) loan
UFFICIALE - Juan @Cuadrado continua la sua avventura in bianconero. https://t.co/8BQUNdeFvC #BentornatoJuan pic.twitter.com/MG3Z3Q76nq
Middlesbrough have confirmed the signing of former Barcelona winger, Adama Traore on a four-year deal.
Marcos Alonso (Fiorentina to Chelsea)
And here he is! Welcome to Chelsea, @marcosalonso03! pic.twitter.com/FX7x6E3nlC
Eliaquim Mangala (Man City to Valencia) loan
OFFICIAL STATEMENT | @Elia22Mangala #welcomeMangala #bienvenueMangala ? https://t.co/PzJRi02UhS pic.twitter.com/IIgIyWt0dV
Serge Gnabri (Arsenal to Werder Bremen) reported fee: €5 million
DONE DEAL: Werder Bremen have signed Serge Gnabry from Arsenal for a reported fee of £4.25m (€5m). pic.twitter.com/oQeLAUKVrA
Samir Nasri (Man City to Sevilla) loan
.@SamNasri19 has joined @SevillaFC on a season-long loan - good luck in La Liga, Samir!https://t.co/9NgFKEUM8k pic.twitter.com/PX0jSuHjgP
— Manchester City (@ManCity) August 31, 2016
Wilfried Bony (Man City to Stoke) loan
BREAKING | #SCFC delighted to announce the season-long loan signing of @wilfriedbony from @ManCity pic.twitter.com/0I0FO81UDd
— Stoke City FC (@stokecity) August 31, 2016
Joe Hart (Man City to Torino) loan
Joe Hart has joined @TorinoFC_1906 on a season-long loan - good luck in Italy, Joe ??
— Manchester City (@ManCity) August 31, 2016
Read: https://t.co/j5HP5FHM0U pic.twitter.com/25aPgYvJ9V
Burnley have completed the signing of Republic of Ireland midfielder, Jeff Hendrick for a club-record fee.
Stoke City have signed Netherlands international Bruno Martins Indi on a season-long loan from FC Porto.
Sunderland have broken their club transfer record to sign Gabon midfielder Didier Ndong for £13.6m from Lorient.
Tuesday's biggest transfers:
Paco Alcacer (Valencia – FC Barcelona) £25.5m
Gabriel Barbosa (Santos – Inter Milan) Undisclosed
Munir El Haddadi (Barcelona – Valencia) Loan
Tomas Rosicky (Arsenal – Sparta Prague) Free
Patrick Bamford (Chelsea – Burnley) Loan
Calum Chambers (Arsenal – Middlesbrough) Loan
Will Keane (Manchester United – Hull City) Undisclosed
Kenedy (Chelsea – Watford) Loan
Shkodran Mustafi (Valencia – Arsenal) About £35m
Lucas Perez (Deportivo La Coruna – Arsenal) £17.1m
Loic Remy (Chelsea – Crystal Palace) Loan
Yevhen Konoplyanka (Sevilla – Schalke) Loan
Read: Top 10 Highest Paid Club Managers In 2016/17
Note: We will update this Deadline Day Transfer Round-Up throughout the day to keep you informed about all the latest news.
Which players would you like to see your club sign today? Have your say in the comments section below.
